Triple-negative breast cancer (TNBC) is a subtype with excessive charges of metastasis, poor prognosis and restricted therapeutic choices. The current research aimed to establish the potential pivotal genes for prognosis and remedy in TNBC. A complete of two microarray expression datasets, GSE38959 and GSE65212, have been downloaded from the Gene Expression Omnibus database, and RNA-sequencing information of breast cancer from The Cancer Genome Atlas database have been analyzed to display screen out differentially expressed genes (DEGs) between TNBC tissues and regular tissues. The intersection of DEGs was submitted to Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses.
A protein-protein interplay (PPI) community was constructed and visualized utilizing Cytoscape software program. Furthermore, module, centrality and survival analyses have been carried out to establish the potential hub genes. Reverse transcription-quantitative (RT-q)PCR evaluation was carried out to detect the expression ranges of key genes in TNBC samples, and 377 DEGs have been recognized. Functional evaluation revealed that the DEGs have been considerably concerned in cell cycle course of, nuclear division and the p53 signaling pathway. A PPI community was constructed with these DEGs, and 66 core genes with excessive centrality options in module 1 have been chosen.
Relapse-free survival evaluation confirmed that top expression ranges of 5 genes [cyclin B1 (CCNB1), GINS complex subunit 2, non-SMC condensin I complex subunit G (NCAPG), minichromosome maintenance 4 (MCM4) and ribonucleotide reductase regulatory subunit M2 (RRM2)] have been considerably related to poor prognosis in TNBC. RT-qPCR evaluation demonstrated that CCNB1, NCAPG, MCM4 and RRM2 have been considerably upregulated in 25 TNBC tissues in contrast with adjoining regular breast tissues. Furthermore, gene set enrichment evaluation revealed that CCNB1, NCAPG, MCM4 and RRM2 have been carefully related to tumor proliferation. Taken collectively, these outcomes recommend that CCNB1, NCAPG, MCM4 and RRM2 are related to tumorigenesis and TNBC development, and thus could act as promising prognostic biomarkers and therapeutic targets for TNBC.
Bioinformatics identification of the candidate microRNAs and development of a competing endogenous RNA regulatory community in lacrimal gland adenoid cystic carcinoma high-grade transformation
Adenoid cystic carcinoma of the lacrimal gland (LACC) is a significant orbital malignancy. The recurrence fee and mortality fee are increased in LACC high-grade transformation (LACC-HGT) in contrast with in LACC. The current research aimed to establish the candidate microRNAs (miRNAs/miRs) and assemble a competing endogenous RNA (ceRNA) regulatory community for LACC-HGT. A miRNA microarray on paraffin-embedded tissues was carried out to establish the differentially expressed miRNAs (DEMs) of LACC-HGT. The overlap with the salivary adenoid cystic carcinoma miRNA/RNA sequencing dataset in the Gene Expression Omnibus was used to establish candidate miRNAs.
In order to assemble a ceRNA regulatory community of LACC-HGT, a microarray of mRNA and circRNA in major cell traces was carried out. The circRNAs and genes with excessive expression in LACC-HGT have been predicted as focusing on miRNAs, and the circRNA-miRNA-mRNA regulatory community was constructed. miR-140-3p was recognized as half of the ceRNA community and as a candidate miRNA, due to this fact this was additional analyzed utilizing reverse transcription-quantitative (RT-q)PCR. Overall, the Agilent Human microarray evaluation recognized a complete of 16 DEMs from the LACC-HGT paraffin-embedded tissues. A complete of 653 DECs and 9,566 DEGs of LACC-HGT major cell traces have been screened through the microarray of mRNA and circRNA.
The ceRNA regulatory community was constructed utilizing the cross-binding of circRNA-miRNA, miRNA-mRNA and the downregulated miRNAs in LACC-HGT to obviously display the circRNA-miRNA-mRNA interplay relationship. RT-qPCR outcomes confirmed that miR-140-3p was downregulated in LACC-HGT tissues and first cell traces in contrast with LACC. Target genes CD200 and parathyroid hormone-related protein have been considerably upregulated in LACC-HGT major cell traces. miR-140-3p and its goal genes could play an vital position in LACC-HGT pathogenesis. In conclusion, the present bioinformatics research constructed a ceRNA community based on a microarray, which can assist establish novel miRNA therapeutic targets for LACC-HGT.
CryptoGenotyper: A brand new bioinformatics instrument for speedy Cryptosporidium identification
Cryptosporidium is a protozoan parasite that’s transmitted to each people and animals by means of zoonotic or anthroponotic means. When a bunch is contaminated with this parasite, it causes a gastrointestinal illness often known as cryptosporidiosis. To perceive the transmission dynamics of Cryptosporidium, the small subunit (SSU or 18S) rRNA and gp60 genes are generally studied by means of PCR evaluation and traditional Sanger sequencing. However, analyzing sequence chromatograms manually is each time consuming and vulnerable to human error, particularly in the presence of poorly resolved, heterozygous peaks and the absence of a validated database.
For this research, we developed a Cryptosporidium genotyping instrument, referred to as CryptoGenotyper, which has the aptitude to learn uncooked Sanger sequencing information for the 2 widespread Cryptosporidium gene targets (SSU rRNA and gp60) and classify the sequence information into normal nomenclature. The CryptoGenotyper has the capability to carry out high quality management and correctly classify sequences utilizing a top quality, manually curated reference database, saving customers’ time and eradicating bias throughout information evaluation. The integrated heterozygous base calling algorithms for the SSU rRNA gene goal resolves double peaks, due to this fact recovering information beforehand categorised as inconclusive.
 BSA Sample Diluent |
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F031A |
Cygnus Technologies |
1000 ml |
EUR 488 |
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Description: BSA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
BSA Sample Diluent |
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F031A-100 |
Cygnus Technologies |
100 ml |
EUR 195 |
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Description: BSA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
BSA Sample Diluent |
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F031A-500 |
Cygnus Technologies |
500 ml |
EUR 411 |
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Description: BSA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
 Sample Diluent Buffer |
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I028-100 |
Cygnus Technologies |
100 ml |
EUR 211 |
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Description: Sample Diluent Buffer by Cygnus Technologies is available in Europe via Gentaur. |
Sample Diluent Buffer |
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I028-1000 |
Cygnus Technologies |
1000 ml |
EUR 519 |
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Description: Sample Diluent Buffer by Cygnus Technologies is available in Europe via Gentaur. |
Sample Diluent Buffer |
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I028-500 |
Cygnus Technologies |
500 ml |
EUR 373 |
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Description: Sample Diluent Buffer by Cygnus Technologies is available in Europe via Gentaur. |
 HEPES Sample Diluent |
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I050 |
Cygnus Technologies |
1000 ml |
EUR 527 |
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Description: HEPES Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
HEPES Sample Diluent |
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I050-100 |
Cygnus Technologies |
100 ml |
EUR 219 |
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Description: HEPES Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
HEPES Sample Diluent |
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I050-500 |
Cygnus Technologies |
500 ml |
EUR 334 |
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Description: HEPES Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
 DNA Sample Diluent |
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D006-100 |
Cygnus Technologies |
100 ml |
EUR 373 |
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Description: DNA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
DNA Sample Diluent |
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D006-1000 |
Cygnus Technologies |
1000 ml |
EUR 1167 |
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Description: DNA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
DNA Sample Diluent |
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D006-500 |
Cygnus Technologies |
500 ml |
EUR 726 |
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Description: DNA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
 Sample diluent buffer |
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AR1106-1 |
BosterBio |
30ml |
EUR 91 |
Sample Diluent Buffer |
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abx296001-20ml |
Abbexa |
20 ml |
EUR 91 |
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 sample) Exosome isolation Solution - Serum/ Plasma (easy version of P101, 95% pure exosome) sample |
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P101EZ--sample |
101Bio |
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 Hemoglobin Sample Diluent, 50ML |
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X052-50ML |
Arbor Assays |
50ML |
EUR 153 |
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X074-12ML |
Arbor Assays |
12ML |
EUR 123 |
, 60ML) Sample Diluent (Conc.), 60ML |
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X074-60ML |
Arbor Assays |
60ML |
EUR 143 |
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F183A |
Cygnus Technologies |
1000 ml |
EUR 835 |
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Description: SP2/0 Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
SP2/0 Sample Diluent |
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F183A-100 |
Cygnus Technologies |
100 ml |
EUR 257 |
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Description: SP2/0 Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
SP2/0 Sample Diluent |
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F183A-500 |
Cygnus Technologies |
500 ml |
EUR 488 |
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Description: SP2/0 Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
 Goat IgG Sample Diluent |
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F213A |
Cygnus Technologies |
1000 ml |
EUR 565 |
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Description: Goat IgG Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
Goat IgG Sample Diluent |
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F213A-100 |
Cygnus Technologies |
100 ml |
EUR 257 |
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Description: Goat IgG Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
Goat IgG Sample Diluent |
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F213A-500 |
Cygnus Technologies |
500 ml |
EUR 411 |
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Description: Goat IgG Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
 NS/0 Sample Diluent |
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F223A |
Cygnus Technologies |
1000 ml |
EUR 835 |
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Description: NS/0 Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
NS/0 Sample Diluent |
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F223A-100 |
Cygnus Technologies |
100 ml |
EUR 257 |
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Description: NS/0 Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
NS/0 Sample Diluent |
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F223A-500 |
Cygnus Technologies |
500 ml |
EUR 488 |
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Description: NS/0 Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
 A549 Sample Diluent, 100mL |
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F233A-100 |
Cygnus Technologies |
100 ml |
EUR 257 |
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Description: A549 Sample Diluent, 100mL by Cygnus Technologies is available in Europe via Gentaur. |
 A549 Sample Diluent, 500mL |
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F233A-500 |
Cygnus Technologies |
500 ml |
EUR 411 |
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Description: A549 Sample Diluent, 500mL by Cygnus Technologies is available in Europe via Gentaur. |
 Neptune Sample Diluent-SD3 |
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KF17354 |
Neuromics |
500 ml |
EUR 244 |
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F243A |
Cygnus Technologies |
1000 ml |
EUR 565 |
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Description: Goat Milk Proteins Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
Goat Milk Proteins Sample Diluent |
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F243A-100 |
Cygnus Technologies |
100 ml |
EUR 257 |
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Description: Goat Milk Proteins Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
Goat Milk Proteins Sample Diluent |
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F243A-500 |
Cygnus Technologies |
500 ml |
EUR 411 |
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Description: Goat Milk Proteins Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
 Mix-N-Go Sample Diluent |
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I600 |
Cygnus Technologies |
25 ml |
EUR 180 |
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Description: Mix-N-Go Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
 Sample Diluent Buffer, 25 mL |
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SDB-025 |
BioAssayWorks |
25 mL |
EUR 121.34 |
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Description: A buffer for dilution of samples in a lateral flow assay. It is for 250 samples. |
 General Purpose Sample Diluent-SD1 |
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KF17352 |
Neuromics |
500 ml |
EUR 291 |
 Standard and Sample Diluent Buffer |
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abx092103-20ml |
Abbexa |
20 ml |
EUR 91 |
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 for ELISA (For serum, plasma sample conservation and low background)) Sample, Antibody and Conjugate Diluent (20X) for ELISA (For serum, plasma sample conservation and low background) |
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80066 |
Alpha Diagnostics |
50 ml |
EUR 232 |
 Mix-N-Go Sample Diluent, 100mL |
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I600-100 |
Cygnus Technologies |
100 ml |
EUR 257 |
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Description: Mix-N-Go Sample Diluent, 100mL by Cygnus Technologies is available in Europe via Gentaur. |
 sample) Exosome isolation Solution - Cell Media/ Urine (easy version of P100, 95% pure exosome) sample |
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P100EZ-sample |
101Bio |
- |
Ask for price |
 Low NSB Sample Diluent for ELISA Kit, ready to use |
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LNSB-30 |
Alpha Diagnostics |
30 ml |
EUR 164 |
Low NSB Sample Diluent for ELISA Kit, ready to use |
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LNSB-500 |
Alpha Diagnostics |
500 ml |
EUR 659 |
 Conjugate Diluent |
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I095 |
Cygnus Technologies |
1 L |
EUR 565 |
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Description: Conjugate Diluent by Cygnus Technologies is available in Europe via Gentaur. |
 Antibody Diluent |
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AR1016 |
BosterBio |
100mL (for 10000-15000 assays) |
EUR 131 |
 Diluent A |
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abx098961-6ml |
Abbexa |
6 ml |
EUR 91 |
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 Diluent B |
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abx098962-12ml |
Abbexa |
12 ml |
EUR 91 |
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 HRP Diluent |
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85-H10S |
Fitzgerald |
250 ml |
EUR 300 |
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Description: Dilution and stabilization buffer for monoclonal or polyclonal antibody HRP conjugates |
 All Sample Diluents Sample Pack |
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KF17355 |
Neuromics |
3X100 ml |
EUR 272 |
 sample/Conjugate Diluent (Green) for ELISA (antibody assay) concentrate (10X)) Whole egg yolk (IgY) sample/Conjugate Diluent (Green) for ELISA (antibody assay) concentrate (10X) |
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80071 |
Alpha Diagnostics |
50 ml |
EUR 202 |
 Substrate Diluent , 12ML |
|
X101-12ML |
Arbor Assays |
12ML |
EUR 128 |
 Conjugate Diluent/HRP |
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I056 |
Cygnus Technologies |
1000 ml |
EUR 565 |
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Description: Conjugate Diluent/HRP by Cygnus Technologies is available in Europe via Gentaur. |
 Antibody diluent buffer |
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AR1106-2 |
BosterBio |
12ml |
EUR 91 |
 ABC diluent buffer |
|
AR1106-3 |
BosterBio |
12ml |
EUR 91 |
 Standard Diluent Buffer |
|
abx098951-20ml |
Abbexa |
20 ml |
EUR 91 |
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 Diluent Buffer A |
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abx296005-10ml |
Abbexa |
10 ml |
EUR 91 |
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 TMB Substrate Diluent |
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42R-TB105 |
Fitzgerald |
1 liter |
EUR 300 |
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Description: Dilution buffer for TMB Substrate in immunoassays |
 General Assay Diluent-AD1 |
|
KF17347 |
Neuromics |
500 ml |
EUR 231 |
 Neptune Assay Diluent-AD3 |
|
KF17349 |
Neuromics |
500 ml |
EUR 244 |
 cDNA Probe Diluent Solution |
|
AR0063 |
BosterBio |
5mL |
EUR 106 |
 HRP Conjugate Stabilizer Diluent |
|
abx090812-1000ml |
Abbexa |
1000 ml |
EUR 662 |
|
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 DB Primary Antibody diluent |
|
DBD-125 |
DB Biotech |
125 ml |
EUR 150 |
DB Primary Antibody diluent |
|
DBD-250 |
DB Biotech |
250 ml |
EUR 245 |
DB Primary Antibody diluent |
|
DB-D-125 |
DB Biotech |
125 ml |
EUR 150 |
DB Primary Antibody diluent |
|
DB-D-250 |
DB Biotech |
250 ml |
EUR 245 |
 Rheumatoid Factor Control Diluent |
|
35-RC15 |
Fitzgerald |
100 ml |
EUR 627 |
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Description: Rheumatoid Factor Control Diluent |
 Universal Casein Diluent/Blocker |
|
85R-108 |
Fitzgerald |
250 ml |
EUR 230 |
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Description: Ready-to-use biotin free reagent applicable for blocking ELISA plates |
 Sample Denaturing Buffer |
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F054 |
Cygnus Technologies |
12 ml |
EUR 195 |
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Description: Sample Denaturing Buffer by Cygnus Technologies is available in Europe via Gentaur. |
 Sample Treatment Buffer |
|
F175 |
Cygnus Technologies |
1000 ml |
EUR 411 |
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Description: Sample Treatment Buffer by Cygnus Technologies is available in Europe via Gentaur. |
Sample Treatment Buffer |
|
F175-100 |
Cygnus Technologies |
100 ml |
EUR 219 |
|
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Description: Sample Treatment Buffer by Cygnus Technologies is available in Europe via Gentaur. |
 Sample Treatment Plate |
|
F402 |
Cygnus Technologies |
1x 96 wells plate |
EUR 103 |
|
|
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Description: Sample Treatment Plate by Cygnus Technologies is available in Europe via Gentaur. |
 IgM Reducing Assay Diluent-AD2 |
|
KF17348 |
Neuromics |
500 ml |
EUR 244 |
 Antigen-Down Assay Diluent-AD4 |
|
KF17350 |
Neuromics |
500 ml |
EUR 282 |
 HRP Stabilizing Diluent, Phosphate Buffered |
|
H3210-010 |
GenDepot |
100ml |
EUR 131 |
HRP Stabilizing Diluent, Phosphate Buffered |
|
H3210-050 |
GenDepot |
500ml |
EUR 232 |
 Universal IHC Diluent/Blocker/Stabilizer |
|
85R-1021 |
Fitzgerald |
1 liter |
EUR 692 |
|
Description: Universal Diluent/Blocker/Stabilizer for use in IHC |
 comb 10 sample, 0.8mm |
|
EVS3100-C10-0.8 |
Consort |
ea |
EUR 59 |
 comb 10 sample, 1.5mm |
|
EVS3100-C10-1.5 |
Consort |
ea |
EUR 59 |
 comb 12 sample, 0.8mm |
|
EVS3100-C12-0.8 |
Consort |
ea |
EUR 59 |
 comb 12 sample, 1.5mm |
|
EVS3100-C12-1.5 |
Consort |
ea |
EUR 59 |
 comb 6 sample, 0.8mm |
|
EVS3100-C6-0.8 |
Consort |
ea |
EUR 59 |
 comb 6 sample, 1.5mm |
|
EVS3100-C6-1.5 |
Consort |
ea |
EUR 59 |
 comb 8 sample, 0.8mm |
|
EVS3100-C8-0.8 |
Consort |
ea |
EUR 59 |
 comb 8 sample, 1.5mm |
|
EVS3100-C8-1.5 |
Consort |
ea |
EUR 59 |
comb 10 sample, 0.8mm |
|
EVS3200-C10-0.8 |
Consort |
ea |
EUR 65 |
comb 10 sample, 1.5mm |
|
EVS3200-C10-1.5 |
Consort |
ea |
EUR 65 |
 comb 15 sample, 0.8mm |
|
EVS3200-C15-0.8 |
Consort |
ea |
EUR 65 |
 comb 15 sample, 1.5mm |
|
EVS3200-C15-1.5 |
Consort |
ea |
EUR 65 |
 comb 20 sample, 0.8mm |
|
EVS3200-C20-0.8 |
Consort |
ea |
EUR 65 |
 comb 20 sample, 1.5mm |
|
EVS3200-C20-1.5 |
Consort |
ea |
EUR 65 |
 comb 25 sample, 0.8mm |
|
EVS3200-C25-0.8 |
Consort |
ea |
EUR 65 |
 comb 25 sample, 1.5mm |
|
EVS3200-C25-1.5 |
Consort |
ea |
EUR 65 |
In this research, in contrast with management specimens, a complete of 708 differentially expressed genes in pediatric sepsis (case-control at a ratio of 1:3) have been recognized, together with 507 up-regulated and 201 down-regulated ones. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment evaluation of differentially expressed genes indicated the shut interplay between neutrophil activation, neutrophil degranulation, hematopoietic cell lineage, Staphylococcus aureus an infection, and periodontitis.
