Increasing DNA Isolation Performance from Blood Cells Using a Modified Commercial Low-Cost Reagent.

Genomic studies facilitate comprehension of the pathophysiology, diagnosis, and treatment of chronic diseases. Such studies require sufficient and good quality DNA isolated from a large number of blood samples.

This study attempts to obtain a high-quality genomic DNA isolated from a large number of blood samples using a simple and cheap method.The EasyPure Genomic DNA Kit (Transgen Biotech) was modified to increase the amount of DNA recovery: a few steps and two additional column elutions were added to the original manufacturer´s procedure.

Individual Reaction Mix 3
G065-3 200 reactions
EUR 167.00

3-D Life Thioglycerol
T10-3 180 µl
EUR 48.00

TGF-b-3 Transforming Growth Factor-Beta 3 Human Recombinant Protein, Plant
PROTP10600-3 Regular: 5ug
EUR 317.00
Description: TGFB3 Human Recombinant produced in plant is a disulfide-linked homodimeric, glycosylated, polypeptide chain containing 118 amino acids and having a molecular mass of 27.2kDa. ;The TGFB3 is fused to 6xHis tag at N-terminus and purified by standard chromatographic techniques.

Dr. P Kit-Solution 3
K2021010-3 50 ml
EUR 133.00
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

pLenti-CARM1 shRNA-3 Plasmid
PVTBAV03691-3 2 ug
EUR 356.00

pLenti-CTLA4 shRNA-3 Plasmid
PVTBAV05689-3 2 ug
EUR 356.00

pLenti-FOXM1 shRNA-3 Plasmid
PVTBAV08732-3 2 ug
EUR 356.00

pLenti-JUN shRNA-3 Plasmid
PVTBAV11741-3 2 ug
EUR 356.00

pLenti-LHX6 shRNA-3 Plasmid
PVTBAV12881-3 2 ug
EUR 356.00

pLenti-MAGEA3 shRNA-3 Plasmid
PVTBAV13661-3 2 ug
EUR 356.00

pLenti-RUNX3 shRNA-3 Plasmid
PVTBAV20583-3 2 ug
EUR 356.00

pLenti-Slc7a11 shRNA-3 Plasmid
PVTBAV21973-3 2 ug
EUR 356.00

pLenti-STAT3 shRNA-3 Plasmid
PVTBAV22921-3 2 ug
EUR 356.00

Recombinant Human MMP-3 Protein
PROTP08254-3 10ug
EUR 317.00
Description: Matrix metalloproteinases (MMPs) are a family of endoproteases that require zinc and calcium for expressing catalytic activity. These enzymes play a central role in the maintenance and remodeling of the extracellular matrix. Elevated expression of their activity, caused either by up-regulation of their expression or down-regulation of their cognate inhibitors, has been implicated in various degenerative disorders, including arthritis, cardiovascular disease, skeletal growth-plate disorders, and cancer metastasis. MMP-3 degrades fibronectin, laminin, collagens III, IV, and X, and cartilage proteoglycans. Recombinant human MMP-3 is a 42.8 kDa protein containing the entire catalytic N-terminal domain and the C-terminal domain (378 amino acids).

3-D Life PEG-Link
L50-3 3x 200 µl
EUR 94.00

3-D Life CD-Link
L60-3 3 x 200 µl
EUR 321.00

3-D-Life SG-PVA
M81-3 3x 170 µl
EUR 148.00

3-D Life FG-PVA
M82-3 3x 170 µl
EUR 148.00

3-D Life SG-Dextran
M91-3 3x 170 µl
EUR 157.00

3-D Life FG-Dextran
M92-3 3x 170 µl
EUR 157.00

3-D Life RGD Peptide
P10-3 3x 1 µmol
EUR 276.00

Anti-VEGF Receptor 3/FLT4 Antibody
A01276-3 100ug/vial
EUR 334.00

Caspase-3 Inhibitor Q-DEVD-OPh
1175-3
EUR 533.00

3-D Life Scrambled RGD Peptide
P11-3 3x 1 µmol
EUR 276.00

FSH (Human Follicle-stimulating hormone) ELISA test
3 96T/Box Ask for price
  • Area of application: Hormone testing
Description: ELISA based test for quantitative detection of FSH (Human Follicle-stimulating hormone)

Anti-active Caspase-3 Rabbit Monoclonal Antibody
M00334-3 100ug/vial
EUR 397.00
Description: Anti-active Caspase-3 Rabbit Monoclonal Antibody tested for IF, IHC, ICC, WB in Human

Anti-Galectin 3/LGALS3 Antibody (monoclonal, 12B12)
M00621-3 100ug/vial
EUR 334.00

QuantiMir 3'-Universal Primer (10,000 qPCR reactions)
RA420AU-3 10,000 assays
EUR 178.00
  • Category: MicroRNA Tools

SeraMir 3'-Universal Primer (10,000 qPCR reactions)
RA800P-3 10,000 reactions
EUR 178.00
  • Category: MicroRNA Tools

Caspase-3 DEVD-R110 Fluorometric HTS Assay Kit
30009-3 1KIT
EUR 809.00
Description: Minimum order quantity: 1 unit of 1KIT

Anti-Glyceraldehyde 3-Phosphate Dehydrogenase [GAPDH] Monoclonal Antibody
M00227-3 100ul
EUR 397.00
Description: Mouse Monoclonal Glyceraldehyde 3-Phosphate Dehydrogenase [GAPDH] Antibody. Validated in IHC, WB and tested in Equine.

3-D Life 10x CB-Buffer (pH 5.5)
B10-3 600 µl
EUR 44.00

3-D Life 10x CB Buffer (pH 7.2)
B20-3 600 µL
EUR 44.00

PLGF3 Human, Placenta Growth Factor-3 Human Recombinant Protein, sf9
PROTP49763-3 Regular: 25ug
EUR 317.00
Description: PLGF3 Human Recombinant produced in Spodoptera frugiperda is a glycosylated homodimer containing 2 chains of 203 amino acids (Leu19-Arg221) and having a molecular mass of 58kDa.;The PLGF-3 is purified by proprietary chromatographic techniques.

ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, cell media, 3 reactions)
M1030-3
EUR 441.00

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, cell media, 3 reactions)
M1031-3
EUR 436.00

TumorExoPure? 0.4 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 3 reactions)
M1032-3
EUR 436.00

TumorExoPure? 1.0 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 3 reactions)
M1033-3
EUR 436.00

MouseExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, mouse biofluids, 3 reactions)
M1034-3
EUR 332.00

MouseExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, mouse biofluids, 3 reactions)
M1035-3
EUR 430.00

MouseExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, mouse cell media, 3 reactions)
M1036-3
EUR 430.00

MouseExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, mouse cell media, 3 reactions)
M1037-3
EUR 430.00

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum, 3 reactions)
M1039-3
EUR 436.00

ANGPTL3 (17-460)Human Angiopoietin-like Protein 3 (17-460 a.a.) Human Recombinant Protein
PROTQ9Y5C1-3 Regular: 10ug
EUR 317.00
Description: ANGPTL3 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 453 amino acids (17-460 a.a.) and having a molecular mass of 52.9kDa (Migrates at 25-70kDa on SDS-PAGE under reducing conditions). 

Lethal factor Protease Substrate 3, AMC derivative MEk2 peptide substrate for high-throughput screening of LF inhibitors (14aa)
LFPS-3 1 mg
EUR 408.00

Mouse Monoclonal anti-PEG (backbone specific) IgG (Clone #3)
PEG13-M-3 100 ug
EUR 482.00

Mouse Monoclonal anti-Clostridium difficile toxin A IgG (Clone #3)
CDTA12-M-3 100 ug
EUR 482.00

Goat Gamma Globulin
22005-3 50g
EUR 1205.00
  • All products from Pel-Freez are available in Europe and worldwide via Gentaur. If you cannot find the item you need send us an inquiry and we will be in touch with you with pricing and delivery information in less than 12 hours.
Description: Goat gamma globulin is purified from serum obtained from normal healthy animals (male and female) by using various fractionation procedures followed by dialysis against low ionic strength buffer. It is in lyophilized powder form with purity of 98% or more. Protein by weight: O.D. 280 nm E1%1.39: ? 85% - Residual salts, lipids, etc. comprise the rest of the weight.

Bovine Serum Sterile, Non-Hemolyzed, Delipidized
37146-3 500mL
EUR 198.00
  • All products from Pel-Freez are available in Europe and worldwide via Gentaur. If you cannot find the item you need send us an inquiry and we will be in touch with you with pricing and delivery information in less than 12 hours.
Description: Whole blood is collected from healthy animals of mixed breed both male and female that are 1+ years of age. The blood is refrigerated within 6 hours after collection and is processed within 24 hours and frozen at -20°C. The serum is then thawed in a cool water bath. First the lipids are removed, then the serum is filtered through a 0.22 micron filter. The obtained product is bottled and frozen at -20 ºC.

Horse Serum Non-Sterile, Non-Hemolyzed
39321-3 500mL
EUR 129.00
  • All products from Pel-Freez are available in Europe and worldwide via Gentaur. If you cannot find the item you need send us an inquiry and we will be in touch with you with pricing and delivery information in less than 12 hours.
Description: Whole blood is collected from both male and female healthy horses living in a herd and are 1+ years of age. The blood is refrigerated within 6 hours after collection and is processed within 24 hours and frozen at -20°C.

Pig Serum, Non-Sterile, Trace-Hemolyzed
39328-3 3L
EUR 356.00
  • All products from Pel-Freez are available in Europe and worldwide via Gentaur. If you cannot find the item you need send us an inquiry and we will be in touch with you with pricing and delivery information in less than 12 hours.
Description: Whole blood is collected from healthy pigs of mixed breed, either sex and fasted at the time of slaughter. The blood is then refrigerated within 6 hours, processed within 24 hours and frozen at -20°C.

Pig Serum, Non-Sterile, Trace-Hemolyzed
39519-3 500mL
EUR 149.00
  • All products from Pel-Freez are available in Europe and worldwide via Gentaur. If you cannot find the item you need send us an inquiry and we will be in touch with you with pricing and delivery information in less than 12 hours.
Description: Whole blood is collected from healthy pigs of mixed breed, either sex and fasted at the time of slaughter. The blood is then refrigerated within 6 hours, processed within 24 hours and frozen at -20°C.

Mouse anti-dsDNA IgG3-specific ELISA Kit, 96 tests, Quantitative
5120-3 1 kit
EUR 712.00

TARGATT? attp Mouse B6 (Age: 36-42 days) *Ship from CRL
549-3 1 female Ask for price
Description: N/A

Anti-PYROXD1 Antibody
A17214-3 100ug/vial
EUR 334.00

Anti-Choline Acetyltransferase/CHAT Antibody
A01192-3 100ug/vial
EUR 334.00

Anti-TAPA1/Cd81 Antibody
A01281-3 100ug/vial
EUR 294.00

Anti-TrkB/NTRK2 Antibody
A01388-3 100ug/vial
EUR 294.00

Anti-PRMT1 Antibody
A01417-3 100ug/vial
EUR 294.00

Anti-CD31/Pecam1 Antibody
A01513-3 100ug/vial
EUR 334.00

Anti-Carbonic Anhydrase 4/CA4 Antibody
A01523-3 100ug/vial
EUR 334.00

Anti-Niemann Pick C2/NPC2 Antibody
A01582-3 100ug/vial
EUR 334.00

Anti-Nag-1 Antibody Peroxidase Conjugated
A01583-3 100ug
EUR 455.00
Description: Rabbit Polyclonal Nag-1 Antibody Peroxidase Conjugated. Validated in IHC, WB and tested in Human, Mouse.

Anti-TFF3 Antibody
A01738-3 100ug/vial
EUR 334.00

Anti-Endothelin A Receptor Antibody
A01828-3 250ug
EUR 484.00
Description: Sheep Polyclonal Endothelin A Receptor Antibody. Validated in IHC and tested in Human, Mouse, Rat.

Anti-GLB1/Beta-galactosidase Antibody
A01829-3 100ug/vial
EUR 334.00

Anti-FPRL1/FPR2 Antibody
A01980-3 100ug/vial
EUR 334.00

Anti-IFNAR2 Antibody
A02056-3 100ug/vial
EUR 334.00

Anti-IL17F Antibody
A02062-3 100ug/vial
EUR 294.00

Anti-TREM1 Antibody
A02135-3 100ug/vial
EUR 294.00

Anti-SENP1 Antibody
A02156-3 100ug/vial
EUR 294.00

Anti-BTC Antibody
A02171-3 100ug/vial
EUR 334.00

Anti-EEA1 Antibody
A02296-3 100ug/vial
EUR 294.00

Anti-Dishevelled 2/DVL2 Antibody
A02404-3 100ug/vial
EUR 294.00

Anti-Alpha 1 microglobulin/AMBP Antibody
A02419-3 100ug/vial
EUR 334.00

Anti-NRAMP1/SLC11A1 Antibody
A02547-3 100ug/vial
EUR 334.00

Anti-CD13/Anpep Antibody
A02591-3 100ug/vial
EUR 334.00

Anti-JAK3 Antibody
A02598-3 100ug/vial
EUR 294.00

Anti-HE4/Wfdc2 Antibody
A02685-3 100ug/vial
EUR 334.00

Anti-APRT Antibody
A02721-3 100ug/vial
EUR 334.00

Anti-B7H4/VTCN1 Antibody
A02821-3 100ug/vial
EUR 294.00

Anti-CUL2 Antibody
A02986-3 100ug/vial
EUR 334.00

Anti-Scramblase 1/Plscr1 Antibody
A02995-3 100ug/vial
EUR 334.00

Anti-GITR/Tnfrsf18 Antibody
A03125-3 100ug/vial
EUR 294.00

Anti-Thrombopoietin/THPO Antibody
A03222-3 100ug/vial
EUR 294.00

Anti-XLF/NHEJ1 Antibody
A03552-3 100ug/vial
EUR 334.00

Anti-HIF3/HIF3A Antibody
A03805-3 100ug/vial
EUR 294.00

Anti-EIF4A1 Antibody
A03922-3 100ug/vial
EUR 334.00

Anti-SLIT1 Antibody
A04113-3 100ug/vial
EUR 294.00

Anti-SORBS1 Antibody
A04426-3 100ug/vial
EUR 334.00

Anti-RNF31/HOIP Antibody
A04457-3 100ug/vial
EUR 294.00

Anti-VEGFB Antibody
A04494-3 100ug/vial
EUR 294.00

Anti-Aldolase/ALDOA Antibody
A05022-3 100ug/vial
EUR 334.00

Anti-SENP6 Antibody
A05088-3 100ug/vial
EUR 294.00

Anti-ZNFN1A2/HELIOS/IKZF2 Antibody
A05228-3 100ug/vial
EUR 294.00

Anti-RAB3GAP2 Antibody
A07244-3 100ug/vial
EUR 334.00

Anti-FOXI1 Antibody
A07512-3 100ug/vial
EUR 334.00

Anti-IL36 alpha/IL36A Antibody
A09802-3 100ug/vial
EUR 334.00

Anti-IL17B Antibody
A10846-3 100ug/vial
EUR 294.00

Anti-RBMS3 Antibody
A12416-3 100ug/vial
EUR 334.00

Anti-TNF alpha Antibody
A00002-3 100ug/vial
EUR 294.00

Anti-p16 Monoclonal Antibody
A00016-3 100ul
EUR 397.00
Description: Mouse Monoclonal Antibody for p16 Antibody (CDKN2A) detection. Tested with WB, IHC in Human, Mouse, Rat.

Anti-APP/C99 Antibody
A00081-3 100ug/vial
EUR 334.00

Anti-IL1 beta Antibody
A00101-3 100ug
EUR 455.00
Description: Rabbit Polyclonal IL1 beta Antibody. Validated in IP, IF, IHC, WB and tested in Mouse, Rat.

Anti-IL-6 Antibody
A00102-3 1mL
EUR 1232.00
Description: Rabbit Polyclonal IL-6 Antibody. Validated in IP, WB and tested in Human.

Anti-Nanog Antibody
A00153-3 100ug/vial
EUR 294.00

Anti-IL17C Antibody
A00164-3 100ug/vial
EUR 294.00

Anti-Flt3/CD135 Antibody
A00188-3 100ug/vial
EUR 334.00

Anti-Aryl hydrocarbon Receptor/Ahr Antibody
A00225-3 100ug/vial
EUR 294.00

Anti-CRP Antibody Fluorescein Conjugated
A00249-3 100ug
EUR 455.00
Description: Goat Polyclonal CRP Antibody Fluorescein Conjugated. Validated in WB and tested in Human.

The amount of DNA isolated from frozen blood samples increased by an average of 56%. Its 260/280 ratio and electrophoretic mobility properties make it suitable for genomic studies.A relatively low-cost commercial column and a simple modification of the manufacturer´s protocol, provided a simple and cheap procedure to isolate high-quality DNA from a large number of blood samples suitable for genomic studies.