Increasing DNA Isolation Performance from Blood Cells Using a Modified Commercial Low-Cost Reagent.

Genomic studies facilitate comprehension of the pathophysiology, diagnosis, and treatment of chronic diseases. Such studies require sufficient and good quality DNA isolated from a large number of blood samples.

This study attempts to obtain a high-quality genomic DNA isolated from a large number of blood samples using a simple and cheap method.The EasyPure Genomic DNA Kit (Transgen Biotech) was modified to increase the amount of DNA recovery: a few steps and two additional column elutions were added to the original manufacturer´s procedure.

Individual Reaction Mix 3
G065-3 200 reactions
EUR 167

3-D Life Thioglycerol
T10-3 180 µl
EUR 48

Western Blot Box - 2 7/8 x 1 3/16 x 3/4in.; 7.3 x 3 x 1.9cm
B1200-3 5/pack
EUR 63.77
Description: Western Blot Boxes

TGF-b-3 Transforming Growth Factor-Beta 3 Human Recombinant Protein, Plant
PROTP10600-3 Regular: 5ug
EUR 317
Description: TGFB3 Human Recombinant produced in plant is a disulfide-linked homodimeric, glycosylated, polypeptide chain containing 118 amino acids and having a molecular mass of 27.2kDa. ;The TGFB3 is fused to 6xHis tag at N-terminus and purified by standard chromatographic techniques.

Dr. P Kit-Solution 3
K2021010-3 50 ml
EUR 133
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

3-D Life RGD Peptide
P10-3 3x 1 µmol
EUR 276

Recombinant Human MMP-3 Protein
PROTP08254-3 10ug
EUR 317
Description: Matrix metalloproteinases (MMPs) are a family of endoproteases that require zinc and calcium for expressing catalytic activity. These enzymes play a central role in the maintenance and remodeling of the extracellular matrix. Elevated expression of their activity, caused either by up-regulation of their expression or down-regulation of their cognate inhibitors, has been implicated in various degenerative disorders, including arthritis, cardiovascular disease, skeletal growth-plate disorders, and cancer metastasis. MMP-3 degrades fibronectin, laminin, collagens III, IV, and X, and cartilage proteoglycans. Recombinant human MMP-3 is a 42.8 kDa protein containing the entire catalytic N-terminal domain and the C-terminal domain (378 amino acids).

3-D-Life SG-PVA
M81-3 3x 170 µl
EUR 148

3-D Life FG-PVA
M82-3 3x 170 µl
EUR 148

3-D Life SG-Dextran
M91-3 3x 170 µl
EUR 157

3-D Life FG-Dextran
M92-3 3x 170 µl
EUR 157

3-D Life PEG-Link
L50-3 3x 200 µl
EUR 94

3-D Life CD-Link
L60-3 3 x 200 µl
EUR 321

pLenti-CARM1 shRNA-3 Plasmid
PVTBAV03691-3 2 ug
EUR 356

pLenti-CTLA4 shRNA-3 Plasmid
PVTBAV05689-3 2 ug
EUR 356

pLenti-FOXM1 shRNA-3 Plasmid
PVTBAV08732-3 2 ug
EUR 356

pLenti-JUN shRNA-3 Plasmid
PVTBAV11741-3 2 ug
EUR 356

pLenti-LHX6 shRNA-3 Plasmid
PVTBAV12881-3 2 ug
EUR 356

pLenti-MAGEA3 shRNA-3 Plasmid
PVTBAV13661-3 2 ug
EUR 356

pLenti-RUNX3 shRNA-3 Plasmid
PVTBAV20583-3 2 ug
EUR 356

pLenti-Slc7a11 shRNA-3 Plasmid
PVTBAV21973-3 2 ug
EUR 356

pLenti-STAT3 shRNA-3 Plasmid
PVTBAV22921-3 2 ug
EUR 356

FSH (Human Follicle-stimulating hormone) ELISA test
3 96T/Box Ask for price
  • Area of application: Hormone testing
Description: ELISA based test for quantitative detection of FSH (Human Follicle-stimulating hormone)

Anti-VEGF Receptor 3/FLT4 Antibody
A01276-3 100ug/vial
EUR 334

Caspase-3 Inhibitor Q-DEVD-OPh
1175-3
EUR 533

3-D Life Scrambled RGD Peptide
P11-3 3x 1 µmol
EUR 276

Anti-active Caspase-3 Rabbit Monoclonal Antibody
M00334-3 100ug/vial
EUR 397
Description: Anti-active Caspase-3 Rabbit Monoclonal Antibody tested for IF, IHC, ICC, WB in Human

Anti-Galectin 3/LGALS3 Antibody (monoclonal, 12B12)
M00621-3 100ug/vial
EUR 334

QuantiMir 3'-Universal Primer (10,000 qPCR reactions)
RA420AU-3 10,000 assays
EUR 178
  • Category: MicroRNA Tools

SeraMir 3'-Universal Primer (10,000 qPCR reactions)
RA800P-3 10,000 reactions
EUR 178
  • Category: MicroRNA Tools

3-D Life 10x CB Buffer (pH 7.2)
B20-3 600 µL
EUR 44

3-D Life 10x CB-Buffer (pH 5.5)
B10-3 600 µl
EUR 44

Caspase-3 DEVD-R110 Fluorometric HTS Assay Kit
30009-3 1KIT
EUR 809
Description: Minimum order quantity: 1 unit of 1KIT

Anti-Glyceraldehyde 3-Phosphate Dehydrogenase [GAPDH] Monoclonal Antibody
M00227-3 100ul
EUR 397
Description: Mouse Monoclonal Glyceraldehyde 3-Phosphate Dehydrogenase [GAPDH] Antibody. Validated in IHC, WB and tested in Equine.

PLGF3 Human, Placenta Growth Factor-3 Human Recombinant Protein, sf9
PROTP49763-3 Regular: 25ug
EUR 317
Description: PLGF3 Human Recombinant produced in Spodoptera frugiperda is a glycosylated homodimer containing 2 chains of 203 amino acids (Leu19-Arg221) and having a molecular mass of 58kDa.;The PLGF-3 is purified by proprietary chromatographic techniques.

ExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, cell media, 3 reactions)
M1030-3
EUR 441

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, cell media, 3 reactions)
M1031-3
EUR 436

TumorExoPure? 0.4 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 3 reactions)
M1032-3
EUR 436

TumorExoPure? 1.0 micron Immunobeads (Tumor-derived Exosome Isolation, biofluids, 3 reactions)
M1033-3
EUR 436

MouseExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, mouse biofluids, 3 reactions)
M1034-3
EUR 332

MouseExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, mouse biofluids, 3 reactions)
M1035-3
EUR 430

MouseExoPure? 0.4 micron Immunobeads (Overall Exosome Isolation, mouse cell media, 3 reactions)
M1036-3
EUR 430

MouseExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, mouse cell media, 3 reactions)
M1037-3
EUR 430

ExoPure? 1.0 micron Immunobeads (Overall Exosome Isolation, plasma, urine, serum, 3 reactions)
M1039-3
EUR 436

ANGPTL3 (17-460)Human Angiopoietin-like Protein 3 (17-460 a.a.) Human Recombinant Protein
PROTQ9Y5C1-3 Regular: 10ug
EUR 317
Description: ANGPTL3 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 453 amino acids (17-460 a.a.) and having a molecular mass of 52.9kDa (Migrates at 25-70kDa on SDS-PAGE under reducing conditions). 

Lethal factor Protease Substrate 3, AMC derivative MEk2 peptide substrate for high-throughput screening of LF inhibitors (14aa)
LFPS-3 1 mg
EUR 408

Mouse Monoclonal anti-PEG (backbone specific) IgG (Clone #3)
PEG13-M-3 100 ug
EUR 482

Mouse Monoclonal anti-Clostridium difficile toxin A IgG (Clone #3)
CDTA12-M-3 100 ug
EUR 482

ABC diluent buffer
AR1106-3 12ml
EUR 91

cDNA from Congenital heart disease: Heart
C1236122Hd-3 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Emphysema: Lung
C1236152Ld-3 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

BioMagPlus Concanavalin A
BP531-3 3 ml
EUR 518.29
Description: BioMagPlus Concanavalin A is magnetic particles with affinity binding proteins may be coated with the primary antibody or biotinylated molecule

Goat anti-mouse IgG (γ chain specific)-HRP
E22NM02-3 100ul
EUR 343

Efficiency Box refill (anti-rabbit)
EB-3 27 vials
EUR 101

Rev-A3-GFP/Luc HIV Reporter Cells
HRC-3 NULL
EUR 0

Hematoxylin, Weigert's Iron Kit
HWI-3 2000 ml
EUR 200

Anti-Human CD45 Monoclonal Antibody Unconjugated Conjugated, Flow Validated
FC00555-3 Various Sizes
EUR 101
Description: Mouse Monoclonal Human CD45 Antibody Unconjugated Conjugated, Flow Validated. Validated in Flow Cytometry and tested in Human.

Empty FPLC 8ml Columns
FPLC8-3 3/pk
EUR 99

Extension of On-going custom antibody Order form
Ext-3 1 Ask for price

Anti-TNF alpha Antibody
A00002-3 100ug/vial
EUR 294

Anti-p16 Monoclonal Antibody
A00016-3 100ul
EUR 397
Description: Mouse Monoclonal Antibody for p16 Antibody (CDKN2A) detection. Tested with WB, IHC in Human, Mouse, Rat.

Anti-APP/C99 Antibody
A00081-3 100ug/vial
EUR 334

Anti-IL1 beta Antibody
A00101-3 100ug
EUR 455
Description: Rabbit Polyclonal IL1 beta Antibody. Validated in IP, IF, IHC, WB and tested in Mouse, Rat.

Anti-IL-6 Antibody
A00102-3 1mL
EUR 1232
Description: Rabbit Polyclonal IL-6 Antibody. Validated in IP, WB and tested in Human.

Anti-Nanog Antibody
A00153-3 100ug/vial
EUR 294

Anti-IL17C Antibody
A00164-3 100ug/vial
EUR 294

Anti-Flt3/CD135 Antibody
A00188-3 100ug/vial
EUR 334

Anti-Aryl hydrocarbon Receptor/Ahr Antibody
A00225-3 100ug/vial
EUR 294

Anti-CRP Antibody Fluorescein Conjugated
A00249-3 100ug
EUR 455
Description: Goat Polyclonal CRP Antibody Fluorescein Conjugated. Validated in WB and tested in Human.

Anti-BMAL1/ARNTL Antibody
A00260-3 100ug/vial
EUR 294

Anti-AICDA Antibody
A00267-3 100ug/vial
EUR 294

Anti-NFkB-p65 (Ab-276) Antibody
A00284-3 100ul
EUR 397
Description: Rabbit Polyclonal NFkB-p65 (Ab-276) Antibody. Validated in IF, IHC, WB and tested in Human, Mouse, Rat.

Anti-ICOS Antibody
A00291-3 100ug/vial
EUR 334

Anti-TNF Receptor I/TNFRSF1A Antibody
A00294-3 100ug/vial
EUR 334

Anti-Interferon gamma/IFNG Antibody
A00393-3 100ug/vial
EUR 334

Anti-TLR1 Antibody
A00429-3 100ug/vial
EUR 334

Anti-IGFBP3 Antibody
A00435-3 100ug/vial
EUR 294

Anti-TANK Antibody
A00445-3 100ug/vial
EUR 334

Anti-beta 2 Microglobulin/B2m Antibody
A00456-3 100ug/vial
EUR 294

Anti-FGF23 Antibody
A00478-3 100ug/vial
EUR 294

Anti-Leptin Antibody
A00479-3 100ug/vial
EUR 294

Anti-CD5 Antibody
A00480-3 100ug/vial
EUR 334

Anti-SYK Antibody
A00490-3 100ug/vial
EUR 294

Anti-PON1 Antibody
A00516-3 100ug/vial
EUR 334

Anti-Cytochrome P450 17A1/Cyp17a1 Antibody
A00615-3 100ug/vial
EUR 334

Anti-CSF1 Antibody
A00620-3 100ug/vial
EUR 294

Anti-Polycystin 2/PKD2 Antibody
A00630-3 100ug/vial
EUR 334

Anti-IL1RA/Il1rn Antibody
A00651-3 100ug/vial
EUR 294

Anti-Smad1 (Ab-465) Antibody
A00728-3 100ul
EUR 397
Description: Rabbit Polyclonal Smad1 (Ab-465) Antibody. Validated in IF, WB and tested in Human, Mouse, Rat.

Anti-LOX 1/Olr1 Antibody
A00760-3 100ug/vial
EUR 334

Anti-Collagen III/COL3A1 Antibody
A00788-3 100ug/vial
EUR 334

Anti-LBP Antibody
A00809-3 100ug/vial
EUR 294

Anti-Fibrinogen alpha chain/FGA Antibody
A00816-3 100ug/vial
EUR 334

Anti-VEGF Receptor 2/Kdr Antibody
A00901-3 100ug/vial
EUR 334

Anti-Cystatin C/Cst3 Antibody
A00961-3 100ug/vial
EUR 294

Anti-IL22 Antibody
A00963-3 100ug/vial
EUR 294

Anti-uPA Receptor/PLAUR Antibody
A00993-3 100ug/vial
EUR 334

Anti-AMPK alpha 1/PRKAA1 Antibody
A00994-3 100ug/vial
EUR 294

Anti-MBL2 Antibody
A01000-3 100ug/vial
EUR 294

Anti-BCMA/Tnfrsf17 Antibody
A01014-3 100ug/vial
EUR 294

Anti-KCNH1 Antibody
A01036-3 100ug/vial
EUR 294

Anti-p90RSK (Ab-348) Antibody
A01058-3 100ul
EUR 397
Description: Rabbit Polyclonal p90RSK (Ab-348) Antibody. Validated in IF, WB and tested in Human, Mouse, Rat.

Anti-RANK/Tnfrsf11a Antibody
A01064-3 100ug/vial
EUR 294

Anti-CD40L/CD40LG Antibody
A01114-3 100ug/vial
EUR 294

Anti-PXR/NR1I2 Antibody
A01133-3 100ug/vial
EUR 334

Anti-IL12B Antibody
A01152-3 100ug/vial
EUR 334

Anti-Choline Acetyltransferase/CHAT Antibody
A01192-3 100ug/vial
EUR 334

Anti-TAPA1/Cd81 Antibody
A01281-3 100ug/vial
EUR 294

Anti-TrkB/NTRK2 Antibody
A01388-3 100ug/vial
EUR 294

Anti-PRMT1 Antibody
A01417-3 100ug/vial
EUR 294

The amount of DNA isolated from frozen blood samples increased by an average of 56%. Its 260/280 ratio and electrophoretic mobility properties make it suitable for genomic studies.A relatively low-cost commercial column and a simple modification of the manufacturer´s protocol, provided a simple and cheap procedure to isolate high-quality DNA from a large number of blood samples suitable for genomic studies.