When working with proteins , the use of impure or low quality samples can lead to the formation of aggregates, precipitates, incorrect folding, loss of activity or even hydrolysis of the same, resulting in inaccurate or low precision experiments. In this post we review some methods to evaluate the purity of our sample and we will see … Read more
Do you know the different formats of purified and unpurified antibodies ? Although the usual thing when buying a commercial antibody is that it comes purified, there are different formats of antibodies available: from raw sera to immunoglobulins purified by different methods. In this post we will see the different presentations in which the antibodies can be supplied for their application in subsequent immunoassays and … Read more
Evaluation of two commercial human T-cell lymphotropic virus western blot (immunoblot) kits with problem specimens.
We evaluated two commercial human T-cell lymphotropic virus (HTLV) Western blot (WB; immunoblot) kits, Cambridge Biotech Corp. (CBC) and Diagnostic Biotechnology Ltd. (DBL). Both strategies make use of HTLV sort I (HTLV-I) viral lysate and rgp21. The DBL WB package additionally distinguishes between HTLV-I and HTLV-II antibodies, utilizing an HTLV-I-specific and an HTLV-II-specific recombinant. Fifty weakly reactive HTLV-II-positive plasma specimens which … Read more
Genetic diversity of Plasmodium falciparum histidine-rich protein 2 (PfHRP2) and its effect on the performance of PfHRP2-based rapid diagnostic tests.
Rapid diagnostic assessments (RDTs) play an important function in the administration and management of malaria an infection. The histidine-rich protein 2 (PfHRP-2) based mostly RDTs are the mostly used RDTs for malaria analysis in Sudan. Deletion of pfhrp2 in Plasmodium falciparum genome have an effect on the accuracy of PfHRP-2 based mostly RDT kits. This examine … Read more
According to the person directions from the producer of OraQuick HIV self-test (HIVST) kits, individuals whose kits present one purple band ought to be thought of to be HIV-negative, regardless of how weak the band is. However, latest studies present potential for a second false weak band after storage, thereby creating confusion in the interpretation of results. In … Read more